rabbit polyclonal anti-gad67  (Thermo Fisher)

Journal: bioRxiv

Article Title: A selective top-down pathway from anterior cingulate cortex embeds a representation of saliency in hippocampus

doi: 10.1101/2025.06.26.661852

Figure Lengend Snippet: (A) Dual retrograde labeling of ACC neurons. Left: The injection sites of retrograde AAVs in RSCd, RSCg and dmSTR. Right: The distribution of fluorescently tagged neurons in the ACC. (B) The proportion of dmSTR-projecting, RSCd-projecting, RSCg-projecting ACC neurons located in ACC layer 2/3 or layer 5. (C) The fraction of ACC projection neurons which project to each area. (D) Example images of sections of ACC labeled with retrograde AAV injected in RSCd/g and immunostained with anti-CaMKIIα or anti-GAD67 antibodies. (E) Similar to (D) but injections of retrograde AAV were targeted to dmSTR. (F) The proportion of RSCd/g-projecting or dmSTR-projecting ACC neurons which were labeled with anti-CaMKIIα or anti-GAD67.

Article Snippet: Prior to staining, slices were blocked in blocking buffer (0.1 M Tris-HCl, 0.15 M NaCl, 0.5% (w/v) blocking reagent (41116100, Roche)) at room temperature (RT) for 1 h. Slices were then washed with TNB-T (0.1 M Tris-HCl (pH 7.5), 0.15 M NaCl, 0.5% (w/v) blocking reagent, 1% Triton (R) X-100) twice for 2 min each and incubated with a monoclonal mouse anti-CaMKIIα antibody (1:250; 6G9, Abcam), or a polyclonal rabbit anti-GAD67 antibody (1:300; GTX 101881), or a monoclonal rabbit anti-c-Fos antibody (1:1,000; 9F6 #2250, Cell Signaling) in TNB-T overnight at 4 °C.

Techniques: Labeling, Injection

Journal: bioRxiv

Article Title: A Novel Role for Phospholamban in the Thalamic Reticular Nucleus

doi: 10.1101/2023.11.22.568306

Figure Lengend Snippet: a) PLN mRNA was detected in the whole brain of adult Pln +/+ mice but not in Pln -/- mice. PLN-specific immunoreactivity is detected in the GABAergic GAD67 + TRN neurons of the adult mouse TRN in b) Pln +/+ but not in c) Pln -/- mice; d) PLN colocalizes with SERCA2 in the TRN neurons of Pln +/+ mice. PLN, GAD67 and SERCA immunoreactivity was assessed using fluorescent IHC in coronal free-floating brain sections from adult Pln +/+ and Pln -/- mice stained with mouse monoclonal PLN (1:100), rabbit polyclonal GAD67 (1:250) and rabbit polyclonal SERCA2 (1:500) primary antibodies. Representative images shown from at least three independent runs were obtained at 200X and 400X magnifications using an Olympus Fluoview 3000 confocal microscope (Olympus, MA, USA).

Article Snippet: The primary antibodies used in our single and/or double IHC experiments included mouse monoclonal PLN (Invitrogen #MA3-922; 1:100), rabbit polyclonal anti-GAD67 (Invitrogen #PA5-21397; 1:250), rabbit polyclonal anti-SERCA2 (Abcam #ab150435; 1:500), and rabbit polyclonal anti-GFAP (Abcam #ab7260, 1:500) and secondary antibodies goat anti-mouse Alexa Fluor 488 (Invitrogen #A32723TR; 1:500), goat anti mouse IgG2a Alexa Fluor 647 (Invitrogen #A21241, 1:500), and goat anti-rabbit Alexa Fluor 568 (Invitrogen #A21428; 1:500).

Techniques: Staining, Microscopy

Journal: bioRxiv

Article Title: A Novel Role for Phospholamban in the Thalamic Reticular Nucleus

doi: 10.1101/2023.11.22.568306

Figure Lengend Snippet: PLN immunoreactivity was not detected in other brain regions, including: a) the prefrontal cortex (PFC); b) the hippocampus (HIPP); c) the striatum (STR); d) the thalamus (TH) and e) the cerebellum (CER). PLN and GAD67 protein expression was assessed using fluorescent IHC in coronal free-floating brain sections from adult Pln +/+ and Pln -/- mice stained with mouse monoclonal PLN (1:100) and rabbit polyclonal GAD67 (1:250) primary antibodies. Representative images were obtained at 200X and 400X magnifications using an Olympus Fluoview 3000 confocal microscope (Olympus, MA, USA).

Article Snippet: The primary antibodies used in our single and/or double IHC experiments included mouse monoclonal PLN (Invitrogen #MA3-922; 1:100), rabbit polyclonal anti-GAD67 (Invitrogen #PA5-21397; 1:250), rabbit polyclonal anti-SERCA2 (Abcam #ab150435; 1:500), and rabbit polyclonal anti-GFAP (Abcam #ab7260, 1:500) and secondary antibodies goat anti-mouse Alexa Fluor 488 (Invitrogen #A32723TR; 1:500), goat anti mouse IgG2a Alexa Fluor 647 (Invitrogen #A21241, 1:500), and goat anti-rabbit Alexa Fluor 568 (Invitrogen #A21428; 1:500).

Techniques: Expressing, Staining, Microscopy